Biography

Dr. Jinzhao Song received his Ph.D. from the Institute of Chemistry, Chinese Academy of Sciences (ICCAS). With over 15 years of experience, he has focused on developing next generation molecular diagnostic systems based on isothermal nucleic acid amplification; microfluidic chip that utilize smartphones for detection, analysis, result recording and sharing; and programmable enzymes such as Argonaute and CRISPR-Cas proteins. He has co-authored 51 peer-reviewed papers with H-index 23, 15 of which he was the lead author, and was named as co-inventor in 9 granted patents and 10 patent applications. Dr. Song is a recipient of the NIH K01 Research Scientist Development Award, as well as a PI/co-PI of an R21 NIH grant and two venture incubation programs. His contributions to the field of molecular diagnostics earned him the AACC's "2019 Young Investigator Award for Outstanding Research in Personalized Medicine." Additionally, Dr. Song founded EzDx Technology Inc., an enterprise dedicated to the commercialization and widespread adoption of these cutting-edge diagnostic tools, thereby setting a new standard in healthcare diagnostics and personalized medicine.

Research Interests

1. IoT Intelligent Molecular Diagnostic Platform: Development of cutting-edge molecular diagnostic systems that integrate Internet of Things (IoT) technology for nucleic acid rapid testing, both at home and in-field. This includes advancements in isothermal nucleic acid amplification methods, smartphone-based detection and analysis platforms, microfluidic chips, and assays utilizing programmable enzymes such as Argonaute and CRISPR-Cas proteins.

2. Disease Early Warning System: The platform leverages real-time data uploads to generate dynamic maps of disease spread, providing immediate visualization of outbreaks. By combining Transformer and Convolutional Neural Network (CNN) technologies, the system boosts efficiency and reliability, enabling rapid responses and precise early warnings. This advancement significantly enhances the effectiveness of disease control measures.

3. Liquid Biopsy: Investigation of liquid biopsy techniques employing programmable enzymes, including Argonaute and CRISPR-Cas proteins, for the precise detection of circulating tumor DNA (ctDNA). The goal is to enhance the accuracy and sensitivity of cancer diagnostics through these advanced methodologies.

4. Cancer Biomarker Discovery: Focus on identifying cancer biomarkers, with particular emphasis on extracellular circular DNA (eccDNA) derived from extracellular vesicles (EVs), to improve early cancer detection and monitoring.

5. Smartphone and AI-Based At-Home Imaging Systems: Development of advanced imaging systems that leverage smartphones and artificial intelligence for at-home applications, such as sperm counting, to provide accessible and accurate diagnostic solutions.

Selected Publications

1. Kaizheng Wang#, Tao Zhang#, Tianjiao Jiao#, Feibiao Pang#, Fengyi Dai#, Zhanfang Zhang, Xiang Li, Yao Chen, Hongyu Zhang, Zhiguang Chen, Jinzhao Song*. Mini-Program Enabled IoT Intelligent Molecular Diagnostic Device for Co-Detection and Spatiotemporal Mapping of Infectious Disease Pathogens. Analytical Chemistry 96, 33, 13494–13503 (2024).
2. Feibiao Pang#, Tao Zhang#, Fengyi Dai#, Kaizheng Wang#, Tianjiao Jiao#, Peng Hu, Jinzhao Song*. A Handheld Isothermal Fluorescence Detection Device for Duplex Visualization of Aquatic Pathogens via Enhanced One-Pot LAMP-PfAgo Assay. Biosensors and Bioelectronics 254, 116187 (2024).
3. Yue Shen, Xiaoling Zhang, Liyi Zhang, Zuoying Zhang, Bao Lyu, Qian Lai, Qinglin Li, Yuhua Zhang, Jieer Ying, Jinzhao Song*. Performance evaluation of a CRISPR Cas9-based selective exponential amplification assay for the detection of KRAS mutations in plasma of patients with advanced pancreatic cancer. Journal of Clinical Pathology,2023/9/7 (2023).
4. Junman Chen#, Tian Qiu#, Michael G. Mauk, Zheng Su, Yaguang Fan, Dennis J. Yuan, Qinghua Zhou, Youlin Qiao, Haim H. Bau, Jianming Ying*, Jinzhao Song*. Programmable endonuclease combined with isothermal polymerase amplification to selectively enrich for rare mutant allele fractions. Chinese Chemical Letters, DOI: 10.1016/j.cclet.2021.11.065 (2022).
5. Junman Chen, Tian Qiu, Michael G. Mauk, Yaguang Fan, Yong Jiang, Jianming Ying, Qinghua Zhou, Youlin Qiao, Haim H. Bau, Jinzhao Song*. CRISPR Cas9-Mediated Selective Isothermal Amplification for Sensitive Detection of Rare Mutant Alleles. Clinical Chemistry, 67, 1569-1571 (2021). 
6. Jinzhao Song*, Mohamed El-Tholoth, Yize Li, Jevon Graham-Wooten, Yining Liang, Juan Li, Weijian Li, Susan R. Weiss, Ronald G. Collman, and Haim H. Bau. Single and Two-Stage, Closed-Tube, Point of Care, Molecular Detection of SARS-CoV-2. Anal. Chem., 93, 13063–13071 (2021).
7. Jinzhao Song*, J.W.H., Michael G. Mauk, Neha Bhagwat, Jacob E. Till, Lotte T. Azink, Jing Peng, Moen Sen, Junman Chen, Jazmine Mays, Erica Carpenter, John van der Oost*, and Haim H. Bau*. Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics. Nucleic Acids Research, 48(4): e19 (2020).
8. Jinzhao Song, Vikram Pandian, Michael G. Mauk, Haim H. Bau, Sara Cherry, Laurence C. Tisic and Changchun Liu. Smartphone-based Mobile Detection Platform for Rapid Molecular Diagnostics and Spatiotemporal Disease Mapping. Anal. Chem., 90, 4823–4831 (2018). (IF=6.32)
9. Jinzhao Song, Changchun Liu, Michael G. Mauk, Jing Peng, Thomas Schoenfeld, and Haim H. Bau*. A Multifunctional Reactor with Dry-Stored Reagents for Enzymatic Amplification of Nucleic Acids. Anal. Chem., 90, 1209–1216 (2018). (IF=6.32)
10. Jinzhao Song, Changchun Liu, Michael G. Mauk, Shelley C. Rankin, Robert M. Greenberg, and Haim H. Bau*. Two-stage isothermal enzymatic amplification for concurrent multiplex molecular detection. Clinical Chemistry, 63, 714-722 (2017).  (IF=8.6)
11. Jinzhao Song, Michael Mauk, Brent Hackett, Sara Cherry, Haim H. Bau and Changchun Liu*. Instrument-Free Point-of-Care Molecular Detection of Zika Virus. Anal. Chem., 88 (14), 7289-7294 (2016). (IF=6.32)
12. Jinzhao Song, Jiangyan Zhang, Fengting Lv*, Yongqiang Cheng, Bing Wang, Liheng Feng, Libing Liu, Shu Wang*. Multiplex detection of DNA mutations by fluorescence fingerprint spectrum technique.Angew. Chem. Int. Ed.52, 13020-13023 (2013). (IF=13.455)
13. Jinzhao Song, Qiong Yang*, Fengting Lv, Libing Liu, Shu Wang*. Visual detection of DNA mutation using multicolor fluorescent coding. ACS Appl. Mater. Interfaces 4, 2885-2890 (2012). (IF=7.504)

Contacts

E-mail: songjinzhao@ucas.ac.cn

Office Room: 4-203

Hangzhou Institute of Medicine

Chinese Academy of Science

150 Fucheng Road, Qiantang District

Hangzhou, Zhejiang Province, China 310000